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首页 产品 iPSC培养试剂
干细胞铺底液
货号：
R2-02-01
规格：
100 mL
储存条件：
-20℃
产品介绍：
干细胞铺底液是使用基质胶配置好的即用型工作液，可以为人多能干细胞（HESC/iPSC）无饲养层培养（feeder-free culture）提供理想的底物支持。
产品优势

即用型铺底液，无需担忧基质胶解冻时间过长或过短造成的性能差异。与iPSC维持培养基联合使用，可以稳定维持人ESC/iPSC在体外培养的多能性、快速增殖能力和正常的核型。

操作流程和注意事项

分装：提前一天在2~8℃℃冰箱中解冻干细胞铺底液；提前准备无菌干燥的移液管或枪头，离心管置于-20℃冰箱预冷。将化冻的干细胞铺底液，置于冰上放入生物安全柜，用预冷的移液管或枪头将铺底液分装到离心管中（按照20mL/支或1~2周使用量分装），并放入-20℃保存。

包板：根据下列表格将细胞铺底液完全覆盖皿底，置于37℃培养箱中孵育2 h后即可使用，或采用parafilm封装后培养板置于4℃冷藏保存，不要超过2周。

6孔板，单孔底面积9.6cm²，用量1mL

12孔板，单孔底面积4.5cm²，用量500µL

24孔板，单孔底面积2cm²，用量300µL

48孔板，单孔底面积1cm²，用量150µL

使用范围
本产品仅供科研使用，不可用于临床治疗等其他用途。
细胞特征
产品数据
产品信息

产品

货号

规格

干细胞铺底液

R2-02-01

100 mL
iPSC培养试剂
其他产品
iPSC维持培养基 EDTA消化液 干细胞铺底液 细胞分化铺底液 神经细胞铺底液
常见问题
iPSC细胞复苏接种时发现大多为单细胞形态，24h后几乎不贴壁，是什么原因，该如何处理？ iPSC细胞传代时的注意事项 细胞出现空泡化 罕见病病人血液iPSC建系的意义
致力于为科研机构、医药企业及临床转化
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  166. /www/wwwroot/somaxbio.com/vendor/topthink/framework/src/think/log/Channel.php ( 3.89 KB )
  167. /www/wwwroot/somaxbio.com/vendor/topthink/framework/src/think/event/LogRecord.php ( 1.02 KB )
  168. /www/wwwroot/somaxbio.com/vendor/symfony/polyfill-mbstring/Mbstring.php ( 35.32 KB )
  169. /www/wwwroot/somaxbio.com/vendor/symfony/polyfill-mbstring/Resources/unidata/lowerCase.php ( 23.96 KB )
  170. /www/wwwroot/somaxbio.com/vendor/topthink/think-helper/src/Collection.php ( 16.47 KB )
  171. /www/wwwroot/somaxbio.com/vendor/topthink/think-orm/src/db/Express.php ( 1.53 KB )
  172. /www/wwwroot/somaxbio.com/vendor/topthink/think-view/src/Think.php ( 8.38 KB )
  173. /www/wwwroot/somaxbio.com/vendor/topthink/framework/src/think/contract/TemplateHandlerInterface.php ( 1.60 KB )
  174. /www/wwwroot/somaxbio.com/vendor/topthink/think-template/src/Template.php ( 46.61 KB )
  175. /www/wwwroot/somaxbio.com/vendor/topthink/think-template/src/template/driver/File.php ( 2.41 KB )
  176. /www/wwwroot/somaxbio.com/vendor/topthink/think-template/src/template/contract/DriverInterface.php ( 0.86 KB )
  177. /www/wwwroot/somaxbio.com/runtime/temp/860317fa64ee7590f085c0fea80e7674.php ( 31.50 KB )
  178. /www/wwwroot/somaxbio.com/app/index/model/LabelModel.php ( 7.63 KB )
  179. /www/wwwroot/somaxbio.com/vendor/topthink/framework/src/think/Response.php ( 8.81 KB )
  180. /www/wwwroot/somaxbio.com/vendor/topthink/framework/src/think/response/Html.php ( 0.97 KB )
  181. /www/wwwroot/somaxbio.com/vendor/topthink/think-trace/src/Html.php ( 4.30 KB )
  1. CONNECT:[ UseTime:0.000568s ] mysql:host=localhost;port=3306;dbname=somaxbio_com;charset=utf8mb4
  2. SHOW FULL COLUMNS FROM `db_cat` [ RunTime:0.001411s ]
  3. SELECT * FROM `db_cat` WHERE `catid` = 21 LIMIT 1 [ RunTime:0.000494s ]
  4. SHOW FULL COLUMNS FROM `db_cat_data` [ RunTime:0.001104s ]
  5. SELECT * FROM `db_cat_data` WHERE `catid` = 21 LIMIT 1 [ RunTime:0.000280s ]
  6. SHOW FULL COLUMNS FROM `db_cms_product` [ RunTime:0.000980s ]
  7. SELECT * FROM `db_cms_product` WHERE `id` = 27 LIMIT 1 [ RunTime:0.000751s ]
  8. SHOW FULL COLUMNS FROM `db_cms_product_data` [ RunTime:0.000840s ]
  9. SELECT * FROM `db_cms_product_data` WHERE `id` = 27 LIMIT 1 [ RunTime:0.000676s ]
  10. SHOW FULL COLUMNS FROM `db_replace` [ RunTime:0.000820s ]
  11. SELECT * FROM `db_replace` ORDER BY `sort` ASC LIMIT 2 [ RunTime:0.000259s ]
  12. UPDATE `db_cms_product` SET `views` = `views` + 1 WHERE `id` = 27 [ RunTime:0.002352s ]
  13. SELECT `title`,`id`,`catid` FROM `db_cms_product` WHERE ( `catid` = 21 AND `id` <> 27 AND `newstime` >= 1769393776 ) ORDER BY `newstime` ASC,`id` ASC LIMIT 1 [ RunTime:0.000696s ]
  14. SELECT `titleurl`,`link` FROM `db_cms_product` WHERE `id` = 28 LIMIT 1 [ RunTime:0.000560s ]
  15. SELECT `title`,`id`,`catid` FROM `db_cms_product` WHERE ( `catid` = 21 AND `id` <> 27 AND `newstime` <= 1769393776 ) ORDER BY `newstime` DESC,`id` DESC LIMIT 1 [ RunTime:0.000694s ]
  16. SELECT `titleurl`,`link` FROM `db_cms_product` WHERE `id` = 26 LIMIT 1 [ RunTime:0.000422s ]
  17. SELECT * FROM `db_cat` WHERE ( `pid` = 7 AND `status` = 1 ) ORDER BY `sort` ASC [ RunTime:0.000522s ]
  18. SELECT `catid`,`modelid` FROM `db_cat` WHERE ( `pid` = 20 AND `status` = 1 ) ORDER BY `sort` ASC [ RunTime:0.000353s ]
  19. SELECT * FROM `db_cms_product` WHERE ( `catid` = 20 ) ORDER BY `sort` ASC [ RunTime:0.001059s ]
  20. SELECT `catid`,`modelid` FROM `db_cat` WHERE ( `pid` = 51 AND `status` = 1 ) ORDER BY `sort` ASC [ RunTime:0.000393s ]
  21. SELECT * FROM `db_cms_product` WHERE ( `catid` = 51 ) ORDER BY `sort` ASC [ RunTime:0.000962s ]
  22. SELECT `catid`,`modelid` FROM `db_cat` WHERE ( `pid` = 21 AND `status` = 1 ) ORDER BY `sort` ASC [ RunTime:0.000638s ]
  23. SELECT * FROM `db_cms_product` WHERE ( `catid` = 21 ) ORDER BY `sort` ASC [ RunTime:0.001030s ]
  24. SELECT `catid`,`modelid` FROM `db_cat` WHERE ( `pid` = 22 AND `status` = 1 ) ORDER BY `sort` ASC [ RunTime:0.000442s ]
  25. SELECT * FROM `db_cms_product` WHERE ( `catid` = 22 ) ORDER BY `sort` ASC [ RunTime:0.001083s ]
  26. SELECT `catid`,`modelid` FROM `db_cat` WHERE ( `pid` = 53 AND `status` = 1 ) ORDER BY `sort` ASC [ RunTime:0.000497s ]
  27. SELECT * FROM `db_cms_product` WHERE ( `catid` = 53 ) ORDER BY `sort` ASC [ RunTime:0.001146s ]
  28. SELECT `catid`,`modelid` FROM `db_cat` WHERE ( `pid` = 41 AND `status` = 1 ) ORDER BY `sort` ASC [ RunTime:0.000437s ]
  29. SELECT * FROM `db_cms_product` WHERE ( `catid` = 41 ) ORDER BY `sort` ASC [ RunTime:0.000990s ]
  30. SELECT * FROM `db_cat` WHERE ( `pid` = 7 AND `status` = 1 ) ORDER BY `sort` ASC,`catid` ASC [ RunTime:0.000492s ]
  31. SELECT `catid`,`modelid` FROM `db_cat` WHERE ( `pid` = 20 AND `status` = 1 ) ORDER BY `sort` ASC [ RunTime:0.000375s ]
  32. SELECT * FROM `db_cms_product` WHERE ( `catid` = 20 ) ORDER BY `sort` ASC [ RunTime:0.000602s ]
  33. SELECT `catid`,`modelid` FROM `db_cat` WHERE ( `pid` = 51 AND `status` = 1 ) ORDER BY `sort` ASC [ RunTime:0.000485s ]
  34. SELECT * FROM `db_cms_product` WHERE ( `catid` = 51 ) ORDER BY `sort` ASC [ RunTime:0.000501s ]
  35. SELECT `catid`,`modelid` FROM `db_cat` WHERE ( `pid` = 21 AND `status` = 1 ) ORDER BY `sort` ASC [ RunTime:0.000381s ]
  36. SELECT * FROM `db_cms_product` WHERE ( `catid` = 21 ) ORDER BY `sort` ASC [ RunTime:0.000394s ]
  37. SELECT `catid`,`modelid` FROM `db_cat` WHERE ( `pid` = 22 AND `status` = 1 ) ORDER BY `sort` ASC [ RunTime:0.000375s ]
  38. SELECT * FROM `db_cms_product` WHERE ( `catid` = 22 ) ORDER BY `sort` ASC [ RunTime:0.000715s ]
  39. SELECT `catid`,`modelid` FROM `db_cat` WHERE ( `pid` = 53 AND `status` = 1 ) ORDER BY `sort` ASC [ RunTime:0.000481s ]
  40. SELECT * FROM `db_cms_product` WHERE ( `catid` = 53 ) ORDER BY `sort` ASC [ RunTime:0.000554s ]
  41. SELECT `catid`,`modelid` FROM `db_cat` WHERE ( `pid` = 41 AND `status` = 1 ) ORDER BY `sort` ASC [ RunTime:0.000378s ]
  42. SELECT * FROM `db_cms_product` WHERE ( `catid` = 41 ) ORDER BY `sort` ASC [ RunTime:0.000458s ]
  43. SELECT `catid`,`modelid` FROM `db_cat` WHERE ( `pid` = 21 AND `status` = 1 ) ORDER BY `sort` ASC [ RunTime:0.000424s ]
  44. SELECT * FROM `db_cms_product` WHERE ( `catid` = 21 ) ORDER BY `sort` ASC [ RunTime:0.000462s ]
  45. SELECT `catid`,`modelid` FROM `db_cat` WHERE ( `pid` = 40 AND `status` = 1 ) ORDER BY `sort` ASC [ RunTime:0.000398s ]
  46. SHOW FULL COLUMNS FROM `db_cms_download` [ RunTime:0.001050s ]
  47. SELECT * FROM `db_cms_download` WHERE ( `catid` = 40 ) ORDER BY `sort` ASC LIMIT 4 [ RunTime:0.000437s ]
  48. SELECT * FROM `db_cat` WHERE ( `pid` = 7 AND `status` = 1 ) ORDER BY `sort` ASC [ RunTime:0.000373s ]
  49. SELECT `catid`,`modelid` FROM `db_cat` WHERE ( `pid` = 41 AND `status` = 1 ) ORDER BY `sort` ASC [ RunTime:0.000312s ]
  50. SELECT * FROM `db_cms_product` WHERE ( `catid` = 41 ) ORDER BY `sort` ASC [ RunTime:0.000510s ]
0.117016s