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首页 产品 iPSC培养试剂
EDTA消化液
货号：
R2-03-01
规格：
100 mL
储存条件：
4℃
产品介绍：
EDTA消化液专为iPSC传代设计，采用无酶温和螯合机制，能最大程度保护细胞活性和多能性。
产品优势

能确保细胞高存活率与规则克隆形态，同时提供标准化操作流程，大幅提升实验可重复性，是维持iPSC最佳状态、确保下游研究可靠性的理想基础工具。

操作流程和注意事项

本产品是即用型，开封后可分装保存，以六孔板一孔为例，吸弃上清，加入1mL PBS清洗，吸弃PBS，每孔加入 1mL EDTA消化液，置于37℃培养箱孵育2~3分钟，在显微镜下观察细胞克隆出现松散裂缝，结束孵育。转移至生物安全柜中吸弃EDTA，吸取1-2mL DMEM/F12，上下各吹打2次细胞，将克隆吹打成10-50细胞的小团块。收集细胞至离心管中以300g，3min离心。

6孔板，单孔底面积9.6cm²，EDTA用量1mL，培养基2mL

12孔板，单孔底面积4.5cm²，EDTA用量500µL，培养基1mL

24孔板，单孔底面积2cm²，EDTA用量300µL，培养基500µL

48孔板，单孔底面积1cm²，EDTA用量150µL，培养基300µL

使用范围
本产品仅供科研使用，不可用于临床治疗等其他用途。
细胞特征
产品数据
产品信息

产品

货号

规格

EDTA消化液

R2-03-01

100 mL
iPSC培养试剂
其他产品
iPSC维持培养基 EDTA消化液 干细胞铺底液 细胞分化铺底液 神经细胞铺底液
常见问题
iPSC细胞复苏接种时发现大多为单细胞形态，24h后几乎不贴壁，是什么原因，该如何处理？ iPSC细胞传代时的注意事项 细胞出现空泡化 罕见病病人血液iPSC建系的意义
致力于为科研机构、医药企业及临床转化
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与解决方案。
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  168. /www/wwwroot/somaxbio.com/vendor/symfony/polyfill-mbstring/Mbstring.php ( 35.32 KB )
  169. /www/wwwroot/somaxbio.com/vendor/symfony/polyfill-mbstring/Resources/unidata/lowerCase.php ( 23.96 KB )
  170. /www/wwwroot/somaxbio.com/vendor/topthink/think-helper/src/Collection.php ( 16.47 KB )
  171. /www/wwwroot/somaxbio.com/vendor/topthink/think-orm/src/db/Express.php ( 1.53 KB )
  172. /www/wwwroot/somaxbio.com/vendor/topthink/think-view/src/Think.php ( 8.38 KB )
  173. /www/wwwroot/somaxbio.com/vendor/topthink/framework/src/think/contract/TemplateHandlerInterface.php ( 1.60 KB )
  174. /www/wwwroot/somaxbio.com/vendor/topthink/think-template/src/Template.php ( 46.61 KB )
  175. /www/wwwroot/somaxbio.com/vendor/topthink/think-template/src/template/driver/File.php ( 2.41 KB )
  176. /www/wwwroot/somaxbio.com/vendor/topthink/think-template/src/template/contract/DriverInterface.php ( 0.86 KB )
  177. /www/wwwroot/somaxbio.com/runtime/temp/860317fa64ee7590f085c0fea80e7674.php ( 31.50 KB )
  178. /www/wwwroot/somaxbio.com/app/index/model/LabelModel.php ( 7.63 KB )
  179. /www/wwwroot/somaxbio.com/vendor/topthink/framework/src/think/Response.php ( 8.81 KB )
  180. /www/wwwroot/somaxbio.com/vendor/topthink/framework/src/think/response/Html.php ( 0.97 KB )
  181. /www/wwwroot/somaxbio.com/vendor/topthink/think-trace/src/Html.php ( 4.30 KB )
  1. CONNECT:[ UseTime:0.000468s ] mysql:host=localhost;port=3306;dbname=somaxbio_com;charset=utf8mb4
  2. SHOW FULL COLUMNS FROM `db_cat` [ RunTime:0.001005s ]
  3. SELECT * FROM `db_cat` WHERE `catid` = 21 LIMIT 1 [ RunTime:0.000346s ]
  4. SHOW FULL COLUMNS FROM `db_cat_data` [ RunTime:0.000931s ]
  5. SELECT * FROM `db_cat_data` WHERE `catid` = 21 LIMIT 1 [ RunTime:0.000372s ]
  6. SHOW FULL COLUMNS FROM `db_cms_product` [ RunTime:0.001081s ]
  7. SELECT * FROM `db_cms_product` WHERE `id` = 30 LIMIT 1 [ RunTime:0.000789s ]
  8. SHOW FULL COLUMNS FROM `db_cms_product_data` [ RunTime:0.000916s ]
  9. SELECT * FROM `db_cms_product_data` WHERE `id` = 30 LIMIT 1 [ RunTime:0.000445s ]
  10. SHOW FULL COLUMNS FROM `db_replace` [ RunTime:0.000959s ]
  11. SELECT * FROM `db_replace` ORDER BY `sort` ASC LIMIT 2 [ RunTime:0.000260s ]
  12. UPDATE `db_cms_product` SET `views` = `views` + 1 WHERE `id` = 30 [ RunTime:0.002296s ]
  13. SELECT `title`,`id`,`catid` FROM `db_cms_product` WHERE ( `catid` = 21 AND `id` <> 30 AND `newstime` >= 1769393824 ) ORDER BY `newstime` ASC,`id` ASC LIMIT 1 [ RunTime:0.000743s ]
  14. SELECT `titleurl`,`link` FROM `db_cms_product` WHERE `id` = 29 LIMIT 1 [ RunTime:0.000563s ]
  15. SELECT `title`,`id`,`catid` FROM `db_cms_product` WHERE ( `catid` = 21 AND `id` <> 30 AND `newstime` <= 1769393824 ) ORDER BY `newstime` DESC,`id` DESC LIMIT 1 [ RunTime:0.000775s ]
  16. SELECT `titleurl`,`link` FROM `db_cms_product` WHERE `id` = 29 LIMIT 1 [ RunTime:0.000352s ]
  17. SELECT * FROM `db_cat` WHERE ( `pid` = 7 AND `status` = 1 ) ORDER BY `sort` ASC [ RunTime:0.000400s ]
  18. SELECT `catid`,`modelid` FROM `db_cat` WHERE ( `pid` = 20 AND `status` = 1 ) ORDER BY `sort` ASC [ RunTime:0.000355s ]
  19. SELECT * FROM `db_cms_product` WHERE ( `catid` = 20 ) ORDER BY `sort` ASC [ RunTime:0.001050s ]
  20. SELECT `catid`,`modelid` FROM `db_cat` WHERE ( `pid` = 51 AND `status` = 1 ) ORDER BY `sort` ASC [ RunTime:0.000421s ]
  21. SELECT * FROM `db_cms_product` WHERE ( `catid` = 51 ) ORDER BY `sort` ASC [ RunTime:0.000947s ]
  22. SELECT `catid`,`modelid` FROM `db_cat` WHERE ( `pid` = 21 AND `status` = 1 ) ORDER BY `sort` ASC [ RunTime:0.000376s ]
  23. SELECT * FROM `db_cms_product` WHERE ( `catid` = 21 ) ORDER BY `sort` ASC [ RunTime:0.000957s ]
  24. SELECT `catid`,`modelid` FROM `db_cat` WHERE ( `pid` = 22 AND `status` = 1 ) ORDER BY `sort` ASC [ RunTime:0.000417s ]
  25. SELECT * FROM `db_cms_product` WHERE ( `catid` = 22 ) ORDER BY `sort` ASC [ RunTime:0.001095s ]
  26. SELECT `catid`,`modelid` FROM `db_cat` WHERE ( `pid` = 53 AND `status` = 1 ) ORDER BY `sort` ASC [ RunTime:0.000484s ]
  27. SELECT * FROM `db_cms_product` WHERE ( `catid` = 53 ) ORDER BY `sort` ASC [ RunTime:0.000985s ]
  28. SELECT `catid`,`modelid` FROM `db_cat` WHERE ( `pid` = 41 AND `status` = 1 ) ORDER BY `sort` ASC [ RunTime:0.000451s ]
  29. SELECT * FROM `db_cms_product` WHERE ( `catid` = 41 ) ORDER BY `sort` ASC [ RunTime:0.000989s ]
  30. SELECT * FROM `db_cat` WHERE ( `pid` = 7 AND `status` = 1 ) ORDER BY `sort` ASC,`catid` ASC [ RunTime:0.000558s ]
  31. SELECT `catid`,`modelid` FROM `db_cat` WHERE ( `pid` = 20 AND `status` = 1 ) ORDER BY `sort` ASC [ RunTime:0.000400s ]
  32. SELECT * FROM `db_cms_product` WHERE ( `catid` = 20 ) ORDER BY `sort` ASC [ RunTime:0.000523s ]
  33. SELECT `catid`,`modelid` FROM `db_cat` WHERE ( `pid` = 51 AND `status` = 1 ) ORDER BY `sort` ASC [ RunTime:0.000388s ]
  34. SELECT * FROM `db_cms_product` WHERE ( `catid` = 51 ) ORDER BY `sort` ASC [ RunTime:0.000505s ]
  35. SELECT `catid`,`modelid` FROM `db_cat` WHERE ( `pid` = 21 AND `status` = 1 ) ORDER BY `sort` ASC [ RunTime:0.000446s ]
  36. SELECT * FROM `db_cms_product` WHERE ( `catid` = 21 ) ORDER BY `sort` ASC [ RunTime:0.000441s ]
  37. SELECT `catid`,`modelid` FROM `db_cat` WHERE ( `pid` = 22 AND `status` = 1 ) ORDER BY `sort` ASC [ RunTime:0.000413s ]
  38. SELECT * FROM `db_cms_product` WHERE ( `catid` = 22 ) ORDER BY `sort` ASC [ RunTime:0.000565s ]
  39. SELECT `catid`,`modelid` FROM `db_cat` WHERE ( `pid` = 53 AND `status` = 1 ) ORDER BY `sort` ASC [ RunTime:0.000477s ]
  40. SELECT * FROM `db_cms_product` WHERE ( `catid` = 53 ) ORDER BY `sort` ASC [ RunTime:0.000418s ]
  41. SELECT `catid`,`modelid` FROM `db_cat` WHERE ( `pid` = 41 AND `status` = 1 ) ORDER BY `sort` ASC [ RunTime:0.000364s ]
  42. SELECT * FROM `db_cms_product` WHERE ( `catid` = 41 ) ORDER BY `sort` ASC [ RunTime:0.000499s ]
  43. SELECT `catid`,`modelid` FROM `db_cat` WHERE ( `pid` = 21 AND `status` = 1 ) ORDER BY `sort` ASC [ RunTime:0.000382s ]
  44. SELECT * FROM `db_cms_product` WHERE ( `catid` = 21 ) ORDER BY `sort` ASC [ RunTime:0.000423s ]
  45. SELECT `catid`,`modelid` FROM `db_cat` WHERE ( `pid` = 40 AND `status` = 1 ) ORDER BY `sort` ASC [ RunTime:0.000489s ]
  46. SHOW FULL COLUMNS FROM `db_cms_download` [ RunTime:0.001016s ]
  47. SELECT * FROM `db_cms_download` WHERE ( `catid` = 40 ) ORDER BY `sort` ASC LIMIT 4 [ RunTime:0.000497s ]
  48. SELECT * FROM `db_cat` WHERE ( `pid` = 7 AND `status` = 1 ) ORDER BY `sort` ASC [ RunTime:0.000407s ]
  49. SELECT `catid`,`modelid` FROM `db_cat` WHERE ( `pid` = 41 AND `status` = 1 ) ORDER BY `sort` ASC [ RunTime:0.000320s ]
  50. SELECT * FROM `db_cms_product` WHERE ( `catid` = 41 ) ORDER BY `sort` ASC [ RunTime:0.000512s ]
0.110546s