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首页 服务 iPSC建系鉴定
iPSC建系鉴定
索码生物提供全球领先的人诱导多能干细胞（iPSC）建系服务，致力于将您珍贵的样本转化为高质量、多能性的研究工具。索码生物拥有成熟稳定的技术平台与大规模的项目经验，对多种来源的样本都可进行建系，已成功构建超过1000株iPSC细胞系，成功率大于99%。建系完成后，我们会提供符合国际标准的iPSC细胞系全套鉴定与质量检测服务。我们的十大检测项目从多能性、遗传稳定性、安全性与功能性等维度进行全面评估，为您交付数据详实、结论明确的权威报告，助您的研究发表无忧。
服务优势
可建系样本来源广泛
我们精通多种体细胞的重编程技术，可针对血液、羊水、皮肤、尿液等多种来源样本进行建系，满足您不同的项目需求。
技术成熟
我们最成熟的方案是针对外周血单个核细胞建系，技术流程经过反复优化与验证，稳定性和效率行业领先。
全国化便捷样本收集网络
为保障样本新鲜度与实验成功率，我们在华东、华北、华南、西北等多地都有专业的PBMC分离站点，可提供全国范围内标准化的样本采集、分离与运输服务，流程十分完善。
严格的质控与完整的交付
构建成功后我们会交付2株单克隆细胞系并附带鉴定报告。
建系流程示意图
服务详情
1、细胞系形态学鉴定：普通光学显微镜，低倍镜，1张 2、AP染色：普通光学显微镜，低倍镜，1张
细胞系形态学鉴定：普通光学显微镜，低倍镜，1张 AP染色：普通光学显微镜，低倍镜，1张

 

3、细胞核型G显带分析：普通光学显微镜，高倍镜，1张 4、支原体检测：PCR鉴定电泳图
细胞核型G显带分析：普通光学显微镜，高倍镜，1张.jpg 支原体检测：PCR鉴定电泳图.jpg

 

5、流式鉴定iPSC细胞特异性标志物比例（SOX2、OCT4、SSEA4、TRA-1-60指标）或免疫荧光多能性基因验证（NANOG, OCT4, SOX2和TRA-1-60指标，高倍镜，每个基因各1张，共4张）。
流式鉴定iPSC细胞特异性标志物比例.jpg
图片 1.jpg

 

6、STR分型检测（PBMC与iPSC可供同时检测，若供体足量）：分型结果图
STR分型检测（PBMC与iPSC可供同时检测，若供体足量）：分型结果图

 

7、外源基因检测分析（质粒电转法：GAPDH、EBNA1/仙台病毒感染法：SEV、KOS、c-Myc指标）：产物凝胶电泳图

 

8、突变位点验证（sanger）：一代测序图片及序列解读结果
突变位点验证（sanger）：一代测序图片及序列解读结果.jpg

 

9、RT-PCR多能基因验证（SOX2、OCT4、NANOG、LIN28和REX1）：结果分析图
RT-PCR多能基因验证（SOX2、OCT4、NANOG、LIN28和REX1）：结果分析图.jpg

 

10、三胚层定向分化（PAX6/NESTIN+SOX17/FOXA2+SMA/BRADRYURY 所有指标均检测）：普通光学显微镜，中高倍镜，每个胚层各1张，共3张
三胚层定向分化（PAX6:NESTIN+SOX17:FOXA2+SMA:BRADRYURY 所有指标均检测）：普通光学显微镜，中高倍镜，每个胚层各1张，共3张.jpg

 

 

服务
iPSC建系鉴定 iPSC基因编辑 iPSC细胞分化 iPSC类器官分化
常见问题
iPSC细胞复苏接种时发现大多为单细胞形态，24h后几乎不贴壁，是什么原因，该如何处理？ iPSC细胞传代时的注意事项 细胞出现空泡化 罕见病病人血液iPSC建系的意义
致力于为科研机构、医药企业及临床转化
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与解决方案。
iPSC产品

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  148. /www/wwwroot/somaxbio.com/vendor/topthink/think-orm/src/db/Connection.php ( 8.39 KB )
  149. /www/wwwroot/somaxbio.com/vendor/topthink/think-orm/src/db/ConnectionInterface.php ( 4.57 KB )
  150. /www/wwwroot/somaxbio.com/vendor/topthink/think-orm/src/db/builder/Mysql.php ( 16.58 KB )
  151. /www/wwwroot/somaxbio.com/vendor/topthink/think-orm/src/db/Builder.php ( 24.06 KB )
  152. /www/wwwroot/somaxbio.com/vendor/topthink/think-orm/src/db/BaseBuilder.php ( 27.50 KB )
  153. /www/wwwroot/somaxbio.com/vendor/topthink/think-orm/src/db/Query.php ( 15.71 KB )
  154. /www/wwwroot/somaxbio.com/vendor/topthink/think-orm/src/db/BaseQuery.php ( 45.13 KB )
  155. /www/wwwroot/somaxbio.com/vendor/topthink/think-orm/src/db/concern/TimeFieldQuery.php ( 7.43 KB )
  156. /www/wwwroot/somaxbio.com/vendor/topthink/think-orm/src/db/concern/AggregateQuery.php ( 3.26 KB )
  157. /www/wwwroot/somaxbio.com/vendor/topthink/think-orm/src/db/concern/ModelRelationQuery.php ( 20.07 KB )
  158. /www/wwwroot/somaxbio.com/vendor/topthink/think-orm/src/db/concern/ParamsBind.php ( 3.66 KB )
  159. /www/wwwroot/somaxbio.com/vendor/topthink/think-orm/src/db/concern/ResultOperation.php ( 7.01 KB )
  160. /www/wwwroot/somaxbio.com/vendor/topthink/think-orm/src/db/concern/WhereQuery.php ( 19.37 KB )
  161. /www/wwwroot/somaxbio.com/vendor/topthink/think-orm/src/db/concern/JoinAndViewQuery.php ( 7.11 KB )
  162. /www/wwwroot/somaxbio.com/vendor/topthink/think-orm/src/db/concern/TableFieldInfo.php ( 2.63 KB )
  163. /www/wwwroot/somaxbio.com/vendor/topthink/think-orm/src/db/concern/Transaction.php ( 2.77 KB )
  164. /www/wwwroot/somaxbio.com/vendor/topthink/framework/src/think/log/driver/File.php ( 5.96 KB )
  165. /www/wwwroot/somaxbio.com/vendor/topthink/framework/src/think/contract/LogHandlerInterface.php ( 0.86 KB )
  166. /www/wwwroot/somaxbio.com/vendor/topthink/framework/src/think/log/Channel.php ( 3.89 KB )
  167. /www/wwwroot/somaxbio.com/vendor/topthink/framework/src/think/event/LogRecord.php ( 1.02 KB )
  168. /www/wwwroot/somaxbio.com/vendor/symfony/polyfill-mbstring/Mbstring.php ( 35.32 KB )
  169. /www/wwwroot/somaxbio.com/vendor/symfony/polyfill-mbstring/Resources/unidata/lowerCase.php ( 23.96 KB )
  170. /www/wwwroot/somaxbio.com/vendor/topthink/think-helper/src/Collection.php ( 16.47 KB )
  171. /www/wwwroot/somaxbio.com/vendor/topthink/think-orm/src/db/Express.php ( 1.53 KB )
  172. /www/wwwroot/somaxbio.com/vendor/topthink/think-view/src/Think.php ( 8.38 KB )
  173. /www/wwwroot/somaxbio.com/vendor/topthink/framework/src/think/contract/TemplateHandlerInterface.php ( 1.60 KB )
  174. /www/wwwroot/somaxbio.com/vendor/topthink/think-template/src/Template.php ( 46.61 KB )
  175. /www/wwwroot/somaxbio.com/vendor/topthink/think-template/src/template/driver/File.php ( 2.41 KB )
  176. /www/wwwroot/somaxbio.com/vendor/topthink/think-template/src/template/contract/DriverInterface.php ( 0.86 KB )
  177. /www/wwwroot/somaxbio.com/runtime/temp/92e9f6eeef64449ae662cdb8b1997999.php ( 26.20 KB )
  178. /www/wwwroot/somaxbio.com/app/index/model/LabelModel.php ( 7.63 KB )
  179. /www/wwwroot/somaxbio.com/vendor/topthink/framework/src/think/Response.php ( 8.81 KB )
  180. /www/wwwroot/somaxbio.com/vendor/topthink/framework/src/think/response/Html.php ( 0.97 KB )
  181. /www/wwwroot/somaxbio.com/vendor/topthink/think-trace/src/Html.php ( 4.30 KB )
  1. CONNECT:[ UseTime:0.000539s ] mysql:host=localhost;port=3306;dbname=somaxbio_com;charset=utf8mb4
  2. SHOW FULL COLUMNS FROM `db_cat` [ RunTime:0.001032s ]
  3. SELECT * FROM `db_cat` WHERE `catid` = 41 LIMIT 1 [ RunTime:0.000422s ]
  4. SHOW FULL COLUMNS FROM `db_cat_data` [ RunTime:0.000964s ]
  5. SELECT * FROM `db_cat_data` WHERE `catid` = 41 LIMIT 1 [ RunTime:0.000292s ]
  6. SHOW FULL COLUMNS FROM `db_cms_product` [ RunTime:0.001124s ]
  7. SELECT * FROM `db_cms_product` WHERE `id` = 43 LIMIT 1 [ RunTime:0.000958s ]
  8. SHOW FULL COLUMNS FROM `db_cms_product_data` [ RunTime:0.001247s ]
  9. SELECT * FROM `db_cms_product_data` WHERE `id` = 43 LIMIT 1 [ RunTime:0.000476s ]
  10. SHOW FULL COLUMNS FROM `db_replace` [ RunTime:0.000870s ]
  11. SELECT * FROM `db_replace` ORDER BY `sort` ASC LIMIT 2 [ RunTime:0.000251s ]
  12. UPDATE `db_cms_product` SET `views` = `views` + 1 WHERE `id` = 43 [ RunTime:0.002419s ]
  13. SELECT `title`,`id`,`catid` FROM `db_cms_product` WHERE ( `catid` = 41 AND `id` <> 43 AND `newstime` >= 1769415859 ) ORDER BY `newstime` ASC,`id` ASC LIMIT 1 [ RunTime:0.000952s ]
  14. SELECT `titleurl`,`link` FROM `db_cms_product` WHERE `id` = 44 LIMIT 1 [ RunTime:0.000455s ]
  15. SELECT `title`,`id`,`catid` FROM `db_cms_product` WHERE ( `catid` = 41 AND `id` <> 43 AND `newstime` <= 1769415859 ) ORDER BY `newstime` DESC,`id` DESC LIMIT 1 [ RunTime:0.000786s ]
  16. SELECT * FROM `db_cat` WHERE ( `pid` = 7 AND `status` = 1 ) ORDER BY `sort` ASC [ RunTime:0.000561s ]
  17. SELECT `catid`,`modelid` FROM `db_cat` WHERE ( `pid` = 20 AND `status` = 1 ) ORDER BY `sort` ASC [ RunTime:0.000360s ]
  18. SELECT * FROM `db_cms_product` WHERE ( `catid` = 20 ) ORDER BY `sort` ASC [ RunTime:0.001042s ]
  19. SELECT `catid`,`modelid` FROM `db_cat` WHERE ( `pid` = 51 AND `status` = 1 ) ORDER BY `sort` ASC [ RunTime:0.000433s ]
  20. SELECT * FROM `db_cms_product` WHERE ( `catid` = 51 ) ORDER BY `sort` ASC [ RunTime:0.000999s ]
  21. SELECT `catid`,`modelid` FROM `db_cat` WHERE ( `pid` = 21 AND `status` = 1 ) ORDER BY `sort` ASC [ RunTime:0.000376s ]
  22. SELECT * FROM `db_cms_product` WHERE ( `catid` = 21 ) ORDER BY `sort` ASC [ RunTime:0.000910s ]
  23. SELECT `catid`,`modelid` FROM `db_cat` WHERE ( `pid` = 22 AND `status` = 1 ) ORDER BY `sort` ASC [ RunTime:0.000369s ]
  24. SELECT * FROM `db_cms_product` WHERE ( `catid` = 22 ) ORDER BY `sort` ASC [ RunTime:0.001170s ]
  25. SELECT `catid`,`modelid` FROM `db_cat` WHERE ( `pid` = 53 AND `status` = 1 ) ORDER BY `sort` ASC [ RunTime:0.000432s ]
  26. SELECT * FROM `db_cms_product` WHERE ( `catid` = 53 ) ORDER BY `sort` ASC [ RunTime:0.000991s ]
  27. SELECT `catid`,`modelid` FROM `db_cat` WHERE ( `pid` = 41 AND `status` = 1 ) ORDER BY `sort` ASC [ RunTime:0.000425s ]
  28. SELECT * FROM `db_cms_product` WHERE ( `catid` = 41 ) ORDER BY `sort` ASC [ RunTime:0.000990s ]
  29. SELECT * FROM `db_cat` WHERE ( `pid` = 7 AND `status` = 1 ) ORDER BY `sort` ASC,`catid` ASC [ RunTime:0.000521s ]
  30. SELECT `catid`,`modelid` FROM `db_cat` WHERE ( `pid` = 20 AND `status` = 1 ) ORDER BY `sort` ASC [ RunTime:0.000317s ]
  31. SELECT * FROM `db_cms_product` WHERE ( `catid` = 20 ) ORDER BY `sort` ASC [ RunTime:0.000436s ]
  32. SELECT `catid`,`modelid` FROM `db_cat` WHERE ( `pid` = 51 AND `status` = 1 ) ORDER BY `sort` ASC [ RunTime:0.000367s ]
  33. SELECT * FROM `db_cms_product` WHERE ( `catid` = 51 ) ORDER BY `sort` ASC [ RunTime:0.000484s ]
  34. SELECT `catid`,`modelid` FROM `db_cat` WHERE ( `pid` = 21 AND `status` = 1 ) ORDER BY `sort` ASC [ RunTime:0.000544s ]
  35. SELECT * FROM `db_cms_product` WHERE ( `catid` = 21 ) ORDER BY `sort` ASC [ RunTime:0.000473s ]
  36. SELECT `catid`,`modelid` FROM `db_cat` WHERE ( `pid` = 22 AND `status` = 1 ) ORDER BY `sort` ASC [ RunTime:0.000307s ]
  37. SELECT * FROM `db_cms_product` WHERE ( `catid` = 22 ) ORDER BY `sort` ASC [ RunTime:0.000493s ]
  38. SELECT `catid`,`modelid` FROM `db_cat` WHERE ( `pid` = 53 AND `status` = 1 ) ORDER BY `sort` ASC [ RunTime:0.000394s ]
  39. SELECT * FROM `db_cms_product` WHERE ( `catid` = 53 ) ORDER BY `sort` ASC [ RunTime:0.000666s ]
  40. SELECT `catid`,`modelid` FROM `db_cat` WHERE ( `pid` = 41 AND `status` = 1 ) ORDER BY `sort` ASC [ RunTime:0.000478s ]
  41. SELECT * FROM `db_cms_product` WHERE ( `catid` = 41 ) ORDER BY `sort` ASC [ RunTime:0.000608s ]
  42. SELECT `catid`,`modelid` FROM `db_cat` WHERE ( `pid` = 41 AND `status` = 1 ) ORDER BY `sort` ASC [ RunTime:0.000370s ]
  43. SELECT * FROM `db_cms_product` WHERE ( `catid` = 41 ) ORDER BY `sort` ASC [ RunTime:0.000417s ]
  44. SELECT `catid`,`modelid` FROM `db_cat` WHERE ( `pid` = 40 AND `status` = 1 ) ORDER BY `sort` ASC [ RunTime:0.000351s ]
  45. SHOW FULL COLUMNS FROM `db_cms_download` [ RunTime:0.000981s ]
  46. SELECT * FROM `db_cms_download` WHERE ( `catid` = 40 ) ORDER BY `newstime` DESC LIMIT 4 [ RunTime:0.000437s ]
  47. SELECT * FROM `db_cat` WHERE ( `pid` = 7 AND `status` = 1 ) ORDER BY `sort` ASC [ RunTime:0.000472s ]
  48. SELECT `catid`,`modelid` FROM `db_cat` WHERE ( `pid` = 41 AND `status` = 1 ) ORDER BY `sort` ASC [ RunTime:0.000385s ]
  49. SELECT * FROM `db_cms_product` WHERE ( `catid` = 41 ) ORDER BY `sort` ASC [ RunTime:0.000568s ]
0.129531s