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首页 产品 iPSC细胞分化试剂盒
iMSC分化试剂盒
货号：
R3-04-01
规格：
1 Kit
储存条件：
基础培液4℃；添加剂-20℃
产品介绍：
iMSC分化试剂盒是由索码生物研发，将人诱导多能干细胞（iPSC）高效转化为间充质干细胞（MSC）。获得的间充质样细胞（iPSC-MSC）在基因表达谱、表面标志物特征和多向分化潜能等方面均与原代MSC高度相似，持续稳定表达CD90、CD73和CD105等MSC标志物，不表达CD45、CD34及HLA-DR等非特异性标志物，并保持成骨、成脂和成软骨的三系分化能力。试剂盒适用于基础和临床科学研究。
产品内容

货号

名称

规格

R3-04-01

iMSC分化培养基A

50 mL

iMSC分化培养基B

200 mL

iMSC维持培养基

200 mL/500 mL
产品优势
采用了先进的小分子定向分化技术，将iPSC高效转化为具有治疗潜力的间充质干细胞（iMSC）
通过精准调控分化过程，获得的间充质样细胞（iMSCs）在基因表达谱、表面标志物特征和多向分化潜能等方面均与原代MSC高度相似
持续稳定表达CD90、CD73和CD105等间充质干细胞标志物；不表达CD45、CD34及HLA等非特异性标志物
保持成骨、成脂和成软骨的三系分化能力
分化流程图

分化流程图.jpg

操作流程和注意事项

储存与解冻：请于指定温度长期保存试剂盒各组分。-20℃组分使用前于4℃缓慢解冻，避免反复冻融，建议根据单次用量分装保存。

使用前检查：使用前请短暂离心并轻柔混匀。如发现沉淀、浑浊或瓶身破损，请勿使用。

严格遵循Protocol：请务必使用试剂盒配套的最新版操作说明书。步骤、时间与试剂比例不得随意调整，以确保分化结果的重现性。

细胞状态要求：仅适用于状态良好、多能性稳定的iPSC细胞系。起始细胞的密度与状态对分化效率至关重要。

专用与纯净：试剂盒各组分专为配套流程设计，请勿与其他体系混用。操作时需使用无菌技术，避免微生物污染。

用途声明：本产品仅供科研用途，不适用于临床诊断、治疗或动物/人体体内应用。

实验设计建议：为保障实验成功，建议首次使用时分批操作并设置细胞密度梯度。

产品数据
CD90
CD73
CD105
CD90阳性
CD73阳性
CD105阳性
阿利新蓝染色（40X）成软骨
茜素红染色（4X）成骨
油红O染色（40X）成脂
iPSC细胞分化试剂盒
其他产品
心肌细胞分化试剂盒 前脑神经元分化试剂盒 NGN2介导神经元分化试剂盒 肾足细胞分化试剂盒 iMSC分化试剂盒 内皮细胞分化试剂盒 脂肪细胞分化试剂盒 iPSC三胚层分化试剂盒
常见问题
iPSC细胞复苏接种时发现大多为单细胞形态，24h后几乎不贴壁，是什么原因，该如何处理？ iPSC细胞传代时的注意事项 细胞出现空泡化 罕见病病人血液iPSC建系的意义
致力于为科研机构、医药企业及临床转化
研究提供一站式的iPSC相关技术、产品
与解决方案。
iPSC产品

iPSC细胞产品

iPSC衍生细胞

iPSC培养试剂

iPSC细胞分化试剂盒

iPSC类器官分化试剂盒

iPSC服务

iPSC建系鉴定

iPSC基因编辑

iPSC细胞分化

iPSC类器官分化

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  155. /www/wwwroot/somaxbio.com/vendor/topthink/think-orm/src/db/concern/TimeFieldQuery.php ( 7.43 KB )
  156. /www/wwwroot/somaxbio.com/vendor/topthink/think-orm/src/db/concern/AggregateQuery.php ( 3.26 KB )
  157. /www/wwwroot/somaxbio.com/vendor/topthink/think-orm/src/db/concern/ModelRelationQuery.php ( 20.07 KB )
  158. /www/wwwroot/somaxbio.com/vendor/topthink/think-orm/src/db/concern/ParamsBind.php ( 3.66 KB )
  159. /www/wwwroot/somaxbio.com/vendor/topthink/think-orm/src/db/concern/ResultOperation.php ( 7.01 KB )
  160. /www/wwwroot/somaxbio.com/vendor/topthink/think-orm/src/db/concern/WhereQuery.php ( 19.37 KB )
  161. /www/wwwroot/somaxbio.com/vendor/topthink/think-orm/src/db/concern/JoinAndViewQuery.php ( 7.11 KB )
  162. /www/wwwroot/somaxbio.com/vendor/topthink/think-orm/src/db/concern/TableFieldInfo.php ( 2.63 KB )
  163. /www/wwwroot/somaxbio.com/vendor/topthink/think-orm/src/db/concern/Transaction.php ( 2.77 KB )
  164. /www/wwwroot/somaxbio.com/vendor/topthink/framework/src/think/log/driver/File.php ( 5.96 KB )
  165. /www/wwwroot/somaxbio.com/vendor/topthink/framework/src/think/contract/LogHandlerInterface.php ( 0.86 KB )
  166. /www/wwwroot/somaxbio.com/vendor/topthink/framework/src/think/log/Channel.php ( 3.89 KB )
  167. /www/wwwroot/somaxbio.com/vendor/topthink/framework/src/think/event/LogRecord.php ( 1.02 KB )
  168. /www/wwwroot/somaxbio.com/vendor/symfony/polyfill-mbstring/Mbstring.php ( 35.32 KB )
  169. /www/wwwroot/somaxbio.com/vendor/symfony/polyfill-mbstring/Resources/unidata/lowerCase.php ( 23.96 KB )
  170. /www/wwwroot/somaxbio.com/vendor/topthink/think-helper/src/Collection.php ( 16.47 KB )
  171. /www/wwwroot/somaxbio.com/vendor/topthink/think-orm/src/db/Express.php ( 1.53 KB )
  172. /www/wwwroot/somaxbio.com/vendor/topthink/think-view/src/Think.php ( 8.38 KB )
  173. /www/wwwroot/somaxbio.com/vendor/topthink/framework/src/think/contract/TemplateHandlerInterface.php ( 1.60 KB )
  174. /www/wwwroot/somaxbio.com/vendor/topthink/think-template/src/Template.php ( 46.61 KB )
  175. /www/wwwroot/somaxbio.com/vendor/topthink/think-template/src/template/driver/File.php ( 2.41 KB )
  176. /www/wwwroot/somaxbio.com/vendor/topthink/think-template/src/template/contract/DriverInterface.php ( 0.86 KB )
  177. /www/wwwroot/somaxbio.com/runtime/temp/902b76982d5c6466b9519844e3d9d29b.php ( 31.63 KB )
  178. /www/wwwroot/somaxbio.com/app/index/model/LabelModel.php ( 7.63 KB )
  179. /www/wwwroot/somaxbio.com/vendor/topthink/framework/src/think/Response.php ( 8.81 KB )
  180. /www/wwwroot/somaxbio.com/vendor/topthink/framework/src/think/response/Html.php ( 0.97 KB )
  181. /www/wwwroot/somaxbio.com/vendor/topthink/think-trace/src/Html.php ( 4.30 KB )
  1. CONNECT:[ UseTime:0.001090s ] mysql:host=localhost;port=3306;dbname=somaxbio_com;charset=utf8mb4
  2. SHOW FULL COLUMNS FROM `db_cat` [ RunTime:0.002644s ]
  3. SELECT * FROM `db_cat` WHERE `catid` = 22 LIMIT 1 [ RunTime:0.000753s ]
  4. SHOW FULL COLUMNS FROM `db_cat_data` [ RunTime:0.001496s ]
  5. SELECT * FROM `db_cat_data` WHERE `catid` = 22 LIMIT 1 [ RunTime:0.000775s ]
  6. SHOW FULL COLUMNS FROM `db_cms_product` [ RunTime:0.002001s ]
  7. SELECT * FROM `db_cms_product` WHERE `id` = 38 LIMIT 1 [ RunTime:0.001175s ]
  8. SHOW FULL COLUMNS FROM `db_cms_product_data` [ RunTime:0.001862s ]
  9. SELECT * FROM `db_cms_product_data` WHERE `id` = 38 LIMIT 1 [ RunTime:0.001331s ]
  10. SHOW FULL COLUMNS FROM `db_replace` [ RunTime:0.001981s ]
  11. SELECT * FROM `db_replace` ORDER BY `sort` ASC LIMIT 2 [ RunTime:0.000543s ]
  12. UPDATE `db_cms_product` SET `views` = `views` + 1 WHERE `id` = 38 [ RunTime:0.003698s ]
  13. SELECT `title`,`id`,`catid` FROM `db_cms_product` WHERE ( `catid` = 22 AND `id` <> 38 AND `newstime` >= 1769393921 ) ORDER BY `newstime` ASC,`id` ASC LIMIT 1 [ RunTime:0.001484s ]
  14. SELECT `title`,`id`,`catid` FROM `db_cms_product` WHERE ( `catid` = 22 AND `id` <> 38 AND `newstime` <= 1769393921 ) ORDER BY `newstime` DESC,`id` DESC LIMIT 1 [ RunTime:0.001504s ]
  15. SELECT `titleurl`,`link` FROM `db_cms_product` WHERE `id` = 37 LIMIT 1 [ RunTime:0.000949s ]
  16. SELECT * FROM `db_cat` WHERE ( `pid` = 7 AND `status` = 1 ) ORDER BY `sort` ASC [ RunTime:0.000882s ]
  17. SELECT `catid`,`modelid` FROM `db_cat` WHERE ( `pid` = 20 AND `status` = 1 ) ORDER BY `sort` ASC [ RunTime:0.000655s ]
  18. SELECT * FROM `db_cms_product` WHERE ( `catid` = 20 ) ORDER BY `sort` ASC [ RunTime:0.000666s ]
  19. SELECT `catid`,`modelid` FROM `db_cat` WHERE ( `pid` = 51 AND `status` = 1 ) ORDER BY `sort` ASC [ RunTime:0.000558s ]
  20. SELECT * FROM `db_cms_product` WHERE ( `catid` = 51 ) ORDER BY `sort` ASC [ RunTime:0.001063s ]
  21. SELECT `catid`,`modelid` FROM `db_cat` WHERE ( `pid` = 21 AND `status` = 1 ) ORDER BY `sort` ASC [ RunTime:0.000471s ]
  22. SELECT * FROM `db_cms_product` WHERE ( `catid` = 21 ) ORDER BY `sort` ASC [ RunTime:0.000850s ]
  23. SELECT `catid`,`modelid` FROM `db_cat` WHERE ( `pid` = 22 AND `status` = 1 ) ORDER BY `sort` ASC [ RunTime:0.000495s ]
  24. SELECT * FROM `db_cms_product` WHERE ( `catid` = 22 ) ORDER BY `sort` ASC [ RunTime:0.000816s ]
  25. SELECT `catid`,`modelid` FROM `db_cat` WHERE ( `pid` = 53 AND `status` = 1 ) ORDER BY `sort` ASC [ RunTime:0.000689s ]
  26. SELECT * FROM `db_cms_product` WHERE ( `catid` = 53 ) ORDER BY `sort` ASC [ RunTime:0.000695s ]
  27. SELECT `catid`,`modelid` FROM `db_cat` WHERE ( `pid` = 41 AND `status` = 1 ) ORDER BY `sort` ASC [ RunTime:0.000888s ]
  28. SELECT * FROM `db_cms_product` WHERE ( `catid` = 41 ) ORDER BY `sort` ASC [ RunTime:0.000612s ]
  29. SELECT * FROM `db_cat` WHERE ( `pid` = 7 AND `status` = 1 ) ORDER BY `sort` ASC,`catid` ASC [ RunTime:0.000443s ]
  30. SELECT `catid`,`modelid` FROM `db_cat` WHERE ( `pid` = 20 AND `status` = 1 ) ORDER BY `sort` ASC [ RunTime:0.000392s ]
  31. SELECT * FROM `db_cms_product` WHERE ( `catid` = 20 ) ORDER BY `sort` ASC [ RunTime:0.001117s ]
  32. SELECT `catid`,`modelid` FROM `db_cat` WHERE ( `pid` = 51 AND `status` = 1 ) ORDER BY `sort` ASC [ RunTime:0.000544s ]
  33. SELECT * FROM `db_cms_product` WHERE ( `catid` = 51 ) ORDER BY `sort` ASC [ RunTime:0.000732s ]
  34. SELECT `catid`,`modelid` FROM `db_cat` WHERE ( `pid` = 21 AND `status` = 1 ) ORDER BY `sort` ASC [ RunTime:0.000477s ]
  35. SELECT * FROM `db_cms_product` WHERE ( `catid` = 21 ) ORDER BY `sort` ASC [ RunTime:0.000793s ]
  36. SELECT `catid`,`modelid` FROM `db_cat` WHERE ( `pid` = 22 AND `status` = 1 ) ORDER BY `sort` ASC [ RunTime:0.000536s ]
  37. SELECT * FROM `db_cms_product` WHERE ( `catid` = 22 ) ORDER BY `sort` ASC [ RunTime:0.000932s ]
  38. SELECT `catid`,`modelid` FROM `db_cat` WHERE ( `pid` = 53 AND `status` = 1 ) ORDER BY `sort` ASC [ RunTime:0.001078s ]
  39. SELECT * FROM `db_cms_product` WHERE ( `catid` = 53 ) ORDER BY `sort` ASC [ RunTime:0.000535s ]
  40. SELECT `catid`,`modelid` FROM `db_cat` WHERE ( `pid` = 41 AND `status` = 1 ) ORDER BY `sort` ASC [ RunTime:0.000608s ]
  41. SELECT * FROM `db_cms_product` WHERE ( `catid` = 41 ) ORDER BY `sort` ASC [ RunTime:0.000962s ]
  42. SELECT `catid`,`modelid` FROM `db_cat` WHERE ( `pid` = 22 AND `status` = 1 ) ORDER BY `sort` ASC [ RunTime:0.000414s ]
  43. SELECT * FROM `db_cms_product` WHERE ( `catid` = 22 ) ORDER BY `sort` ASC [ RunTime:0.000821s ]
  44. SELECT `catid`,`modelid` FROM `db_cat` WHERE ( `pid` = 40 AND `status` = 1 ) ORDER BY `sort` ASC [ RunTime:0.000663s ]
  45. SHOW FULL COLUMNS FROM `db_cms_download` [ RunTime:0.001890s ]
  46. SELECT * FROM `db_cms_download` WHERE ( `catid` = 40 ) ORDER BY `sort` ASC LIMIT 4 [ RunTime:0.000517s ]
  47. SELECT * FROM `db_cat` WHERE ( `pid` = 7 AND `status` = 1 ) ORDER BY `sort` ASC [ RunTime:0.000485s ]
  48. SELECT `catid`,`modelid` FROM `db_cat` WHERE ( `pid` = 41 AND `status` = 1 ) ORDER BY `sort` ASC [ RunTime:0.000510s ]
  49. SELECT * FROM `db_cms_product` WHERE ( `catid` = 41 ) ORDER BY `sort` ASC [ RunTime:0.000877s ]
0.181240s